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Becker Hickl GmbH
Technology Leader Photon Counting
Technology Leader Photon Counting
About Founded 1993, Becker Hickl have introduced proprietary time-correlated single-photon counting principle that made TCSPC more than times faster than existing devices. Moreover, introduced multi-dimensional TCSPC process that records photons only versus time signal period, also versus other parameters, such experiment time, wavelength, spatial coordinates. devices designed record multi-dimensional photon distributions, time-resolved images, sequences photon distributions, multi-dimensional time-tag data. TCSPC products complemented picosecond diode lasers, detector modules, multi-spectral detector assemblies, experiment control modules. Based these components Becker Hickl supply their confocal fluorescence lifetime laser scanning microscope FLIM upgrade kits laser scanning microscopes various manufacturers. Moreover, supplier TCSPC modules commercial time-domain optical tomography instruments. market activities include currently workshops around world distribution more than 1200 pages TCSPC literature.
1993 1994 1995 1996 1996 1997 1998 1999 2000 Introduction multidimensional TCSPC. Introduction fast TAC/ADC conversion. TCSPC becomes times faster than before. SPC300 modules with simultaneously recording detector channels. Time channel width Saturated count rate MHz. SPC-330 modules. Improved width. Routers parallel operation detectors. SPC-430 modules. Continuous Flow mode infinite sequential recording. Saturated count rate MHz. First single-molecule applications. Resolution bursts, lifetime analysis within bursts. SPC-432 modules. Introduction Time-Tag recording single molecule spectroscopy. First version PML-16 sixteen channel module SPC-535 modules. First implementation TCSPC FLIM. First FLIM applications. PMS-300 gated photon counter multichannel scaler SPC-630 modules. Introduction bus. Modules contain time-tag mode, standard photon distribution modes, continuous flow mode. PMH-100 detector module simplifies system setup. SPC-730 modules. Introduction TCSPC FLIM. Scan Sync Scan Sync mode. First applications FLIM microscopy. MSA-300 multichannel scaler. count rate, channel width. SPC-134 four-channel packages optical tomography. Devices contain continuous flow mode, standard photon distribution modes, time-tag mode. Saturated sustained count rate MHz. SPC-130 TCSPC board industrial applications. FLIM upgrade kits Zeiss microscopes. SPCImage FLIM data analysis software. MSA-1000 multichannel scaler. resolution, count rate 1000 MHz. BHL-600 picosecond diode lasers. SPCM software introduces autosave, auto-accumulate, automatic page stepping functions SPC-830 modules. Fast interface. Saturated sustained count rate MHz. Standard photon distribution modes, FLIM modes, time-tag recording. first time, FLIM single-molecule techniques were combined instrument. DCC-100 detector controller solves problem detector overload. PMC-100 cooled detector modules. Internal preamplifier, internal high-voltage generator, overload shutdown. System setup greatly simplified. Detector-Shutter modules non-descanned ports multiphoton microscopes. FLIM systems Leica, Olympus, Biorad laser scanning microscopes. First multi-spectral FLIM BHLP-700 picosecond diode lasers. BDL-405 picosecond diode lasers. DDG-100 pulse generator multiplexed operation lasers modules. First demonstration double-exponential FRET imaging. SPC-144 four-channel TCSPC Imaging Package. Saturated sustained count rate MHz. BDL-375, 440, picosecond diode lasers PML-16 multi-wavelength detector Online FCS, PCH, trace calculation time-tag mode TCSPC modules Simple-Tau Simple-Tau compact TCSPC systems. MW-FLIM, first multi-spectral FLIM systems multiphoton microscopes. Macro-time synchronisation several TCSPC modules. First full correlation down picosecond region. PML-16 sixteen channel detector. Internal high-voltage generator, overload shutdown, control DCC-100. High-efficiency single-mode fibre coupling picosecond diode lasers FLIM upgrade kits confocal laser scanning microscopes based BDL-SM lasers TCSPC technology. Becker, Advanced Time-Correlated Single Photon Counting Techniques published Springer. First edition TCSPC Handbook. pages, references. High-power mode BDL-SMC picosecond diode lasers. Second edition TCSPC Handbook. pages, references. DCS-120 confocal laser scanning systems. Complete fluorescence-lifetime laser scanning microscopes. DPC-230 channel photon correlator, resolution. SPC-154 package SPC-150 modules. Standard photon distribution modes, continuous flow mode, TCSPC imaging, continuous-flow imaging, imaging time-tag mode. Imaging time-tag mode SPC-140 SPC-830.
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catalog 2007
Technology Leader Photon Counting
Modular TCSPC systems
SPC-630 TCSPC module
Multi-dimensional photon distribution mode Photon stream (time tag) mode; buffer size photons Multi-detector multi-wavelength operation combination with fluorescence lifetime Burst-integrated fluorescence lifetime detection Fast unlimited sequential recording Fast multiplexing excitation sources Total recordable count rate MHz, saturated count rate Time channel width Each channel expandable detectors 16-channel multi-wavelength detection with PML-SPEC assembly
SPC-830 TCSPC module
Multi-dimensional photon distribution mode Photon stream (time tag) mode; buffer size photons Multi-detector multi-wavelength operation Synchronisation channels photon correlation Fast scanning: single- multi-wavelength FLIM module laser scanning microscopes combination with fluorescence lifetime Burst-integrated fluorescence lifetime detection Fast sequential recording Fast multiplexing excitation sources Total recordable count rate MHz, saturated count rate Time channel width Each channel expandable detectors 16-channel multi-wavelength detection with PML-SPEC assembly
SPC-134 TCSPC package
Four fully parallel TCSPC channels Multi-dimensional photon distribution mode Photon stream (time tag) mode; buffer size photons Multi-detector multi-wavelength operation Synchronisation channels photon correlation combination with fluorescence lifetime Burst-integrated fluorescence lifetime detection Unlimited fast sequential recording: TCSPC module systems Fast multiplexing excitation sources Total recordable count rate Each channel expandable detectors
SPC-144 TCSPC package
Four fully parallel TCSPC channels Multi-dimensional photon distribution mode Photon stream (time tag) mode; buffer size photons Synchronisation channels photon correlation Fast scanning: High-speed FLIM module laser scanning microscopes combination with fluorescence lifetime Burst-integrated fluorescence lifetime detection Fast sequential recording Fast multiplexing excitation sources Total recordable count rate Each channel expandable detectors
New!
SPC-154 TCSPC package
Four fully parallel TCSPC channels Multi-dimensional photon distribution mode Photon stream (time tag) mode; buffer size photons Synchronisation channels photon correlation Fast scanning: High-speed FLIM module laser scanning microscopes combination with fluorescence lifetime Burst-integrated fluorescence lifetime detection Unlimited fast sequential recording: TCSPC module systems Fast multiplexing excitation sources Total recordable count rate Each channel expandable detectors
Simple-Tau compact TCSPC systems
Recording SPC-140 SPC-830 TCSPC module Detector control DCC-100 detector controller card Compact FLIM systems laser scanning microscopy General time-resolved fluorescence spectroscopy Single-molecule spectroscopy Combined fluorescence lifetime systems Burst-integrated fluorescence lifetime experiments
New!
Simple-Tau compact TCSPC systems
parallel SPC-150 TCSPC channels DCC-100 detector controller Portable systems High-Speed FLIM systems
catalog 2007
Technology Leader Photon Counting
SPC-130, SPC-140, SPC-150 TCSPC Modules Industrial Applications
Standard fluorescence lifetime, TCSPC FLIM, Diffuse optical tomography, Singlemolecule spectroscopy Rugged design Overvoltage-protected inputs Photon distribution, time-tag, sequential-recording imaging modes price large order volumes
Picosecond Photon Correlators New!
Correlator
Recording absolute photon times Fluorescence correlation down times combined with fluorescence decay Autocorrelation within LVTTL channels 16-channel multiscaler mode 3-channel reversed-start-stop TCSPC mode
DPC-230 Channel Picosecond Photon
Multichannel Scalers
MSA-1000 Multichannel Scaler
Recording luminescence decay functions range Time-of-flight mass spectroscopy Lidar Time resolution time channel Maximum count rate Compact Simple-Tau systems available
MSA-300 Multichannel Scaler
Recording luminescence decay functions range Time-of-flight mass spectroscopy Lidar Time resolution time channel Maximum count rate Compact Simple-Tau systems available
PMS-400 Gated Photon Counter Multichannel Scaler
Dated detection optical signals Luminescence decay range Chemoluminescence parallel recording channels Gating down Multiscaler operation down channel
Detectors Detector Assemblies
Detector shutter assemblies
PMC-100 detector: width Hamamatsu R3809U PMTs: width Hamamatsu H7422 modules: width ultra-high efficiency Shutter operation DCC-100 detector controller Overload shutdown Input field lens Compatible with ports multiphoton laser scanning microscopes Part modular FLIM systems
Shutter beamsplitter detector assemblies
PMC-100, R3809U H7422 detectors Shutter operation DCC-100 detector controller Overload shutdown Dichroic beamsplitters, Input field lens, input filter, bandpass filters front detectors Compatible with ports multiphoton laser scanning microscopes Part modular FLIM systems
PMC-100 cooled modules
Cathode versions region Internal voltage generation width Ultra-stable recorded count rates Internal preamplifier Overload shutdown Power supply control DCC-100 detector controller Part modular FLIM modular systems
Single-photon avalanche photodiode modules (SPADs)
Ultra-high efficiency visible region width Active areas 20µm, 50µm 100µm Stable over count rate Detector count rates MHz, recorded count rates
catalog 2007
Technology Leader Photon Counting
New!
PML-SPEC multi-wavelength detection modules
Simultaneous fluorescence decay detection wavelength channels Multi-spectral FLIM confocal laser scanning microscopes width Works with TCSPC modules Internal high-voltage power supply Internal routing electronics Overload shutdown Full control DCC-100 card Part multi-wavelength FLIM systems laser scanning microscopes
FLIM multi-spectral FLIM detection systems multiphoton microscopes
Multi-spectral FLIM Non-descanned (direct) detection Adapters Zeiss, Leica, Olympus Efficient light collection from deep sample layers width Works with TCSPC modules Internal high-voltage power supply Internal routing electronics Protected shutter overload shutdown Full control DCC-100 card Part multi-wavelength FLIM systems laser scanning microscopes
New!
Detector Electronics
HRT-41 four-channel router
Connects four PMC-100, H7422 R3809U detectors TCSPC channel detectors detecting simultaneously
HRT-81 eight-channel router
Connects eight PMC-100, H7422 R3809U detectors TCSPC channel detectors detecting simultaneously
HRT-82 eight-channel router
Connects eight SPAD modules TCSPC channel detectors detecting simultaneously
HFAC-26 HFAM-26 Preamplifiers
R3809U PMT, H7422 modules, other PMTs Power supply from TCSPC module Overload shutdown conjunction with DCC-100 controller
Experiment Control
DCC-100 detector controller
Power supply, gain control overload shutdown detectors Control shutters other actuators Control detector shutter assemblies Power control BHLP lasers Part modular FLIM, modular DOT, Simple-Tau systems
DDG-200 Digital Delay Generator
Multiplexing lasers routing into TCSPC Modules Multiplexing rate Non-overlapping multiplexing On-times programmable individually Part modular systems
New!
GVD-120 Scanner Control Card
Generation scan signals galvo mirrors piezo stages Dual-axis control Fully digital signal generation Independent software response times Cycloid flyback Extremely high scan rates Pixel times down Fully Integrated into SPCM software TCSPC modules Part DCS-120 confocal scanning systems
catalog 2007
Technology Leader Photon Counting
Picosecond Diode Lasers
BDL-375-SMC, BDL-405-SMC, BDL-440-SMC BDL-473-SMC picosecond diode lasers
Single-mode fibre coupler with beam correction optics coupling efficiency commonly used fibre couplers with mounting pitch Repetition rate High-Power mode Pulse width typically Fast on/off/multiplexing capability Fast electronic power control driver electronics integrated Simple wall mounted +12V power supply from single-mode fibre 440, from single-mode fibre Part modular FLIM systems laser scanning microscopes
New!
BDL-375-SMCP, BDL-405-SMCP, BDL-440-SMCP BDL-473-SMCP picosecond diode lasers with fibre pigtails
Single-mode fibre pigtail Single-mode fibre permanently attached laser fibre alignment required
New!
BHL-600 picosecond diode lasers
Wavelengths from 1300 Repetition rate Pulse width typ. Power typ. driver electronics integrated Simple wall mounted +12V power supply
BHLP-700 picosecond diode lasers
Wavelengths from Repetition rate Pulse width typ. power Power driver electronics integrated Fast on/off/multiplexing capability Power control DCC-100 detector controller driver electronics integrated Simple wall mounted +12V power supply Part modular systems
catalog 2007
Technology Leader Photon Counting
Fluorescence Lifetime Microscopy New!
Confocal Scanning Systems
FLIM upgrade conventional microscopes Fully confocal Scanning fast galvano mirrors input channels BDL-SMC picosecond diode lasers Laser wavelengths High excitation power beam-profile-corrected laser couplers detection channels Channel separation dichroic polarising beamsplitters Individually selectable pinholes Individually selectable filters High efficiency direct-coupled detectors PMC-100, H7422 ultra-fast detector modules 16-channel multi-wavelength detector module Compatible with SPC-830, SPC-140, SPC-150 TCSPC modules Software Windows 2000, Vista
FLIM Systems Zeiss Multiphoton Microscopes
Non-descanned detection detectors Multi-spectral FLIM detection Detectors connected Zeiss switch options light path available Zoom functions available Works scan rate Ultra-high time resolution: with detector Fast Acquisition Full overload protection detectors SPC-830 SPC-150 TCSPC channels based systems Simple-Tau Controlled Zeiss conjunction with SPCM software
FLIM Systems Zeiss Confocal Microscopes
Excitation BDL-405 picosecond diode laser Confocal detection Single wavelength detection Multi-spectral detection Zoom functions available Works scan rate Fast Acquisition Overload protection detectors based systems Simple-Tau Controlled Zeiss conjunction with SPCM software
FLIM Systems Leica Microscopes
TCSPC technique fully integrated into Leica systems Confocal detection Direct (RLD) detection multiphoton microscopes Single wavelength detection Multi-spectral detection capability Zoom functions available Works scan rate Wavelength scan function applicable Fast Acquisition Full overload protection detectors Controlled Leica conjunction with SPCM software
FLIM Systems Olympus FV1000 FV300
Confocal FV1000 systems: Excitation BDL-405 BDL-473 picosecond diode laser High efficiency direct coupling detectors Single-wavelength multi-wavelength detection capability Multiphoton FV1000 FV300 systems: High efficiency non-descanned FLIM detection modules Single-wavelength multi-wavelength detection Full overload protection detectors Zoom functions FV1000 FV300 available Works scan rate
catalog 2007
Technology Leader Photon Counting
Time-Domain Diffuse Optical Tomography
Modular systems
Several BHLP-700 lasers different wavelengths multiplexed Time-of -flight distributions four wavelength channels four spatial channels Four parallel detectors Four parallel TCSPC channels DDG-200 digital delay generator SPC-154 SPC-134 TCSPC packages Detector control DCC-100 cards Saturated count rates Multiplexing periods lasers individually programmable Unlimited sequential recording continuous flow mode Acquisition rate time-of-flight distributions second
1100 Pages TCSPC Literature from Technology Leader
Wolfgang Becker Advanced Time-Correlated Single Photon Counting Techniques pages, figures, references Springer 2005, ISBN 3-540-26047-1
Wolfgang Becker TCSPC Handbook Second Edition, 2006 pages, figures, references Available from www.becker-hickl.com
Modular FLIM Systems Zeiss Laser Scanning Microscopes pages, figures, references Available from www.becker-hickl.com
Leica MP-FLIM D-FLIM Fluorescence Lifetime Microscopy Systems pages, figures, references Available from www.becker-hickl.com
Modular FLIM Systems Olympus Laser Scanning Microscope Fluo View FV1000 pages, figures, references Available from www.becker-hickl.com
BDL-SMC Blue Picosecond Diode Lasers User Handbook pages, figures, references Available from www.becker-hickl.com
PML-16-C Channel Detector Head Time-Correlated Single Photon Counting User Handbook pages, figures, references Available from www.becker-hickl.com
catalog 2007
Technology Leader Photon Counting
Software
SPCM Data Acquisition Software TCSPC modules
software TCSPC modules Full access functions boards Parallel operation four modules Online display images curves Online calculation FCS, PCH, traces Page stepping Accumulation autosave functions Oscilloscope mode Single repeat mode Sequential modes Imaging modes Time mode Time imaging mode Integrated scanner control display modes Curve mode, colour-intensity mode Software gating image display Display multi-wavelength FLIM images Saving, loading, conversion photon distributions, images, time-tag data Loading predefined setups data processing functions Main panel configurable user Automatic interaction with SPCImage FLIM data analysis Emulation mode working without hardware Runs under Windows 2000, Vista SPCM software free. downloaded from www.becker-hickl.com time.
SPCImage FLIM Data Analysis Software
Deconvolution single curves FLIM data Single, double, triple-exponential decay analysis Single double-exponential FRET Display lifetime images Display FRET images Display lifetimes, amplitudes, intensities ratios these parameters Histograms lifetimes, amplitudes, intensities ratios these parameters Export lifetime data Export images Direct interaction with SPCM software
catalog 2007
Technology Leader Photon Counting
Operating Software Multichannel Scalers
Setting system parameters Configuration measurement sequences Data display Data processing Load save measurement data setup parameters Operation four modules Emulation mode work without hardware Runs under windows 2000, Vista software free. downloaded from www.beckerhickl.com time.
Operating Software DCC-100 Detector controller
Safe operation PMTs, Modules, MCPs Control high voltage modules high voltage power supplies Control shutters Control coolers panel placed anywhere within SPCM software panels Runs under windows 2000, Vista software free. downloaded from www.becker-hickl.com time.
View Libraries TCSPC Multiscaler Modules
Integration systems into user-designed instrument software
catalog 2007
Technology Leader Photon Counting
Design, Manufacturing Quality Management
Strong Partnership
Dorazil form alliance since 1993. Located same building, companies closely cooperate stages through schematics design, hybrid circuit design, board layout, computer-aided manufacturing testing. Continuous quality management through complete design manufacturing flow results high reliability short delivery time both prototypes large order quantities.
Continuos flow from design into manufacturing
Hybrid-circuit, FPGA, design
Electronics running mill
High-density automatic placement Automatic soldering ROHS lead-free
Thin film thick film hybrid cricuits
In-house manufacturing Automatic placement components Automatic chip bonding
9000 certified
Quality management though whole manufacturing process Uncompromised quality number pieces
catalog 2007
Becker Hickl GmbH Nahmitzer Damm 12277 Berlin, Berlin Tel. Fax. email: info@becker-hickl.com www.becker-hickl.com

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